Soil Nitrate Reductase (NR) Activity Assay Kit

Note: Take two or three different samples for prediction before test.

Operation Equipment: Spectrophotometer/ Microplate reader

Cat No: AK0369

Size ：100T/48S

Product Description:

S-NR catalyzes the reduction of nitrate to nitrite in soil, which is the key enzyme of nitrate reduction in soil.  Study on the activity of S-NR is of great  significance for rational fertilization and reduction of nitrogen loss.

 

S-NR catalyzes the reduction of nitrate to nitrite, NO3-+NADH+H+→NO2-+NAD++H2O; the generated nitrite  can  quantitatively  generate  red  azo  compounds  with  p-aminobenzenesulfonic  acid  and  α- naphthylamine under acidic conditions; The unreacted NADH will inhibit the subsequent color reaction, and then carry out the subsequent reaction with PMS; the generated red azo compounds are 520 nm has a maximum absorption peak, which can be determined by spectrophotometry.

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Totalgoods,subtotals:￥Checkout