Serum Total Iron Binding Capacity (TIBC) Assay Kit

Note: Take two or three different samples for prediction before test.

Operation Equipment: Spectrophotometer/ Microplate reader

Cat No: AK0373

Size: 100T/96S

Description:

Total iron-binding capacity (TIBC) refers to the ability of serum transferrin to bind iron, and its content is closely related to the diseases such as iron deficiency anemia and acute hepatitis.Fe2+ reacts with ferrozine to form a fuchsia compound which has an absorption peak at 562nm. In alkaline condition, serum transferrin can bind with Fe3+, and the remaining unbound Fe3+  can be reduced to Fe2+ . So the absorbance A1 is positively correlated with Fe3+ . After acidification, the transferrin-bound Fe3+  is released and further reduced to Fe2+ . The absorbance A2 has a positive correlation with Fe3+, A2 minus A1 was proportional to TIBC.

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