Glutamic-oxalacetic Transaminase (GOT) Activity Assay Kit

Detection equipment: Spectrophotometer/microplate reader

Note: Take two or three different samples for prediction before test.

 

Cat No: AK0420

 

Size: 100T/48S

Product Description:

GOT is widely found in animals, plants, microbes and cultured cells. It catalyzes the reversal of amino reactions  and is  an important  enzyme in  amino  acid metabolism. In  addition,  GOT is the highest in cardiomyocytes  and  is  commonly  used  as  an  assisted  examination  of  myocardial  infarction  and myocarditis in clinical practice. The serum concentration of liver damage can also be increased.

 

GOT catalyze  α-ketoglutaric acid react with aspartate to produce glutamic acid and oxaloacetic acid. Oxaloacetic   acid   is   further   decarboxylated   to   form   pyruvate,   pyruvate   can   react   with   2,   4- dinitrophenylhydrazine to produce  2,4-dinitrophenylhydrazone, which  shows brownish red  in  alkaline condition, the activity of GOT enzyme activity can be calculated by measuring the absorbance of 505 nm.

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