Glutamic-pyruvic transaminase  (GPT) Activity Assay Kit 

Note: Take two or three different samples for prediction before test.

Operation Equipment: Spectrophotometer/ microplate reader

 

Cat No: AK0422

 

Size: 100T/48S

Product Description:

GPT is widely found in animals, plants, microbes and cultured cells, which is an important enzyme in amino acid metabolism. It catalyzes the transamination of amino acid and keto acid. In addition, GPT activity is very high in mammalian liver cells. GPT is released into the blood when liver cells necrotic, serum  GPT  activity is  significantly increased. Therefore,  GPT is recommended  as the most  sensitive indicator of liver damage by the World Health Organization.

 

GPT  catalyzes  the  transamination  reaction  of alanine  and  α-ketoglutarate  to  generate  pyruvate  and glutamic acid; the addition of 2,4-dinitrophenylhydrazine solution not only terminates the above reaction, but  also  increases  Into phenylpyrene pyruvate;  which  shows brownish  red  in  alkaline  condition,  the activity of GOT enzyme activity can be calculated by measuring the absorbance of 505 nm.

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