Diamine Oxidase(DAO) Activity Assay Kit

Note: Take two or three different samples for prediction before test.

Operation Equipment: Spectrophotometer

Catalog Number: AK0458

Sizes ：50T/24S

Product Description：

Diamine oxidase (DAO, EC1.4.3.6) widely exists in animals (intestinal mucosa, lung, liver, kidney, etc.), plants and microorganisms. It can catalyze the oxidation of polyamines to aldehydes, and its activity is closely related to the synthesis of nucleic acids and proteins. It can reflect the integrity and damage degree of intestinal mechanical barrier.

 

DAO catalyzes the production of aldehydes and hydrogen peroxide from cadaverine, and it can catalyze the oxidation of o-dianisidine by hydrogen peroxide to generate colored substances with adding excessive horseradish peroxidase. This colored substance has a characteristic absorption at 500 nm. The activity of DAO can be calculated by measuring the absorbance increase rate of this wavelength.

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