NAD Kinase (NADK) Activity Assay Kit

Note: Take two or three different samples for prediction before the formal determination. 

Detection equipment: Spectrophotometer/Microplate

Cat No: AK0499

Size:100T/48S

Description:

NADK (EC 2.7.1.23) is widely found in animals, plants, microorganisms and cultured cells. It is the only enzyme that can catalyze the phosphorylation of NAD+  to NADP+  in organisms. It can catalyze the phosphorylation reaction of NAD(H) with ATP or inorganic polyphosphate [poly(P)] as a phosphoryl donor  to  generate  NADP(H).  Therefore,  NAD  kinase  plays  an  important  role  in  the  synthesis  of NADP(H) and the regulation of the balance between NAD(H) and NADP(H).

NADK catalyzes the phosphorylation of NAD+ to produce NADP+, which can be reduced to NADPH by glucose-6-phosphate dehydrogenase. NADPH could reduce oxidized thiazole blue (MTT) through the dehydrogenation of PMS. The absorbance value of MTT at 570 nm can be reflect the activity of NADK.

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