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γ-glutamylcysteine Ligase (GCL) Activity Assay Kit
γ-glutamylcysteine Ligase (GCL) Activity Assay Kit
γ-谷氨酰半胱氨酸连接酶(GCL)活性检测试剂盒
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γ-Glutamylcysteine Ligase(GCL) Activity Assay Kit

Detection equipment: Spectrophotometer/Microplate reader

Note: Take two or three different samples for prediction before test.

Cat No:AK0471

Size:100T/48S

Description

GCL(Glutamylcysteineligase) is the rate limiting enzyme of GSH synthesis. GSH has feedback inhibition to GCL. GCL gene express manipulated by many factors, such as oxidants, antioxidants, growth factors and inflammatory  factors. The  activity of GCL has important influence to  GSH content  and ratio of

GSH/GSSG.

 

In the presence of ATP and Mg2+, GCL catalyzes the synthesis of γ-glutamyl cysteine from glutamate and cysteine, and Dephosphorylation of ATP produce inorganic phosphorus molecules. The activity of GCL can be calculated by measuring the increasing rate of inorganic phosphorus.

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