Thioredoxin Reductase (TrxR) Activity Assay Kit

Note: Take two or three different samples for prediction before test.

Operation Equipment: Spectrophotometer/Microplate Reader

Cat No: AK0481

Size:100T/96S

Product Description:

TrxR is a NADPH-dependent dimer selenase and includes FAD structure domain. TrxR belongs to pyridine  nucleotide-disulfide  REDOX  enzyme,  and  form  thioredoxin  system  with  thioredoxin  and NADPH. The activity of TrxR is similar with GR. TrxR could catalyzes GSSG reduce to GSH, which is the key enzyme in glutathione REDOX cycle.

TrxR catalyzes NADPH to reduceDTNB form TNB and NADP+, TNB has a absorbance at 412 nm, but reduced glutathione  reacts  with  DTNB  to  form  TNB,  so  the  2-Vinylpyridine  in  this  kit  can  inhibit reduced glutathione in sample, the activity of TrxR can be calculated by detecting increase rate of TNB at

 

412 nm.

 

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