NADP Malic Enzyme(NADP-ME) Activity Assay Kit

Note: Take two or three different samples for prediction before test.

Detection equipment:Spectrophotometer

Cat No:AK0486

Size:50T/48S

Description:

ME widely exist in microbe, culturing cells, animal and plant cytoplasm. Especially it has high activity in plant  tissue.  ME  catalyzesthe  reversible  reaction  of malic  acidoxidative  decarboxylation  to  produce pyruvic  acid  and  CO2with  the  reduction  of NAD(P)+,  which  is  the  crucial  enzyme  of malic  acid metabolism.  The  activity  of ME  is  related with biosynthesis  and  anti-oxidation.  In  recent years,  the determination of plant ME activity become a hot spot in antioxidant research. According the coenzyme specificity    and    substrate    specificity,    MEis    divided    into   NAD-ME(EC1. 1. 1.38)    and   NADP- ME(EC 1.1.1.40).

 

NADP-ME catalyzesthe reduction of NADP+ to NADPH,detect the increase rate of NADPH at 340nm.

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