Pyruvate Kinase  (PK) Activity Assay Kit

Note: Take two or three different samples for prediction before test.

Operation Equipment: Spectrophotometer/Microplate reader

Catalog Number: AK0539

Size:100T/96S

Product Description

Pyruvate Kinase (PK, EC 2.7. 1.40) is widely exists in animals, plants, microorganisms and cultured cells. It could catalyze the final step of the glycolysis process. PK is one of the major rate-limiting enzymes in the glycolysis process and one of the key enzymes for ATP production. Therefore, the determination of PK activity is of great significance.

PK  catalyzes  the  generation  of  ATP  and  pyruvate  from  phosphoenolpyruvate  and  ADP.  Lactate dehydrogenase  further  catalyze NADH  and pyruvate  to  generate  lactic  acid  and NAD+ .  The NADH degradation rate can measured at 340 nm to reflect the activity of PK.

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