Mitochondrial Respiratory Chain Complex Ⅰ Activity Assay Kit

Note: Take two or three different samples for prediction before test.

Operation Equipment: Spectrophotometer/Microplate reader

Cat No: AK0543

Size:100T/96S

Product Description:

Mitochondrial  Respiratory  Chain  Complex  Ⅰ  (EC  1.6.5.3)  same  as NADH-CoQ  reductase  or NADH dehydrogenase, exists widely in mitochondria of animals, plants, microorganisms and cultured cells, which is the largest protein complex in inner mitochondrial membrane. This enzyme catalyzes a pair of electrons transfer from NADH to CoQ, reduce O2 to O2-, which is the main site of O2- production on the respiratory electron  transport  chain.  The  enzyme  activity  of Complex  Ⅰ  reflects  the  state  of respiratory  electron transport chain (ETC) and produce state of ROS.

 

Complex Ⅰ catalyzes NADH to form NAD+, the activity of this enzyme can be calculated by detecting the oxidation rate of NADH at 340 nm.

 

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