Carboxylesterase (CarE) Activity Assay Kit

Note: Take two or three different samples for prediction before test.

Detection equipment: Spectrophotometer/Microplate reader

Cat No: AK0513

Size: 100T/96S

Description:

Mammalian  CarE  also  known  as  aliphatic  esterase  (aliesterase)  is  widely  distributed  in  tissue  and organism,  belonging  to  the  serine  hydrolase  family.  CarE  catalyze  hydrolysis  of  endogenous  and exogenous  substances  containing  ester  bonds,  amide  bonds,  and  thioester  bonds,  but  can’t  catalyze hydrolysis of acetylcholine and its analogues. CarE take part in lipid transport and metabolism, and related with   detoxification   and   metabolism   of   many   drugs,   environmental   poisons   and   carcinogens. Organophosphorus pesticides can bind to CarE and inhibit CarE activity.

 

CarE  can  catalyze  acetic  acid- 1-naphthalene  ester  to  produce  naphthalene  ester,  solid  blue  color development. Determination of 450 nm light absorption increase rate could calculate CarE activity.

 

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