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Lactate Dehydrogenase (LDH) Activity Assay Kit
Note: Take two or three different samples for prediction before test.
Operation Equipment: Spectrophotometer/microplate reader
Cat No: AK0523
Size:100T/48S
Product Description:
Lactate dehydrogenase (LDH or LD) is the terminal enzyme of the glycolysis pathway which is found in nearly all living cells (animals, plants, and prokaryotes). LDH catalyzes the conversion of lactate to pyruvic acid and back, as it converts NAD+ to NADH and back.
NAD+ and lactic acid are oxidized to pyruvic acid by the catalysis of LDH. Pyruvate further reacted with 2,4-dinitrophenylhydrazide to form pyruvate dinitrobenzone, which show brown red color in alkaline solution and the color depth is proportional to the concentration of pyruvate.
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