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Detection Device: Microplate reader Application: TG is a fat molecule formed by long-chain fatty acids and glycerol, which is not only the main component of cell membrane, but also an important respiratory substrate. The TG, extracted with isopropyl alcohol and saponified with KOH, then form glycerol and fatty acids by hydrolysis. glycerol was oxidized by periodic acid to form formaldehyde, which reacts with diacetone form yellow components in presence of chloride ions, the color depth is proportional to the TG content. There is a characteristic absorption at 420 nm.
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