Triglyceride(TG) Content Assay Kit

Note: Take two or three different samples for prediction before test.

Operation Equipment: Spectrophotometer

Catalog Number: AK0530

Size:50T/48S

Product Description:

 

Triglyceride(TG) is a fat molecule formed by long-chain fatty acids and glycerol, which is not only the main component of cell membrane, but also an important respiratory substrate. The TG is extracted with isopropyl alcohol, then hydrolysis to glycerol and fatty acids after saponification of TG by KOH. Glycerol is oxidized by periodic acid to form formaldehyde. Condensation of formaldehyde and acetylacetone to form  yellow  components  in  presence  of chloride  ions.  The  yellow  component  has  a  characteristic absorption at 420 nm and proportional to the TG content.

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