Detection Device:

Microplate reader

Application:

PDH is widely exist in animals, plants, microorganism and cultured cells, which is the rate-limiting enzyme of acetylformic acid oxidative and decarboxylate catalyzed by Pyruvate dehydrogenase complex (PDHC). The decarboxylation of acetylformic acid forms hydroxyethyl -TPP, links glycolysis to the three carboxylic acid cycle. PDH catalyzes the dehydrogenation of acetylformic acid and reduct 2, 6-dichlorophenol indophenol (2,6-DCPIP), which makes the absorption of 600 nm decrease.

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