Coenzyme Ⅰ NAD(H) Content Assay Kit

Note ：It is necessary to predict 2-3 large difference samples before the formal determination. Operation Equipment: Spectrophotometer

Cat No: AK0560

Size: 50T/24S

Product Description：

Coenzyme I include both reduced and oxidized forms and plays a role in hydrogen transfer in biological oxidation.  Oxidized  coenzyme  I  also  called nicotinamide  adenine  dinucleotide  (NAD+) which  is  the coenzyme of dehydrogenase. It is an important role in glycolysis, gluconeogenesis, tricarboxylic acid cycle and  respiratory  chain.  Intermediate  product  will  transfer  hydrogen  to NAD  make  it  become NADH (reduced  coenzyme  I). NADH  acts  as  a  carrier  for hydrogen  and  synthesizes  ATP by  chemosmosis coupling in the respiratory chain. NADH has important physiological significance in the body. It is closely related to substance metabolism, energy metabolism, anti-cell aging, anti-oxidation and the occurrence of some diseases. A decrease in coenzyme I levels in the body can lead to cell damage or death.

Extract the sample of NAD+  and NADH with acidic and alkaline extract solution respectively. NADH reduces the oxidized Thiazolyl Blue Tetrazolium Blue (MTT) to form formazan by hydrogen transfer from PMS, formazan has characteristic absorption at 570 nm. NAD could be reduced to NADH by alcohol dehydrogenase. Further, MTT reduction method was used to detect NAD+ .

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