Ketone body Assay Kit

Note: Take two or three different samples for prediction before test.

Operation Equipment: Ultraviolet spectrophotometer/Microplate reader

Catalog Number: AK0003

Size: 100T/96S

Product Description：

Ketone  bodies  are  intermediate  products  of fatty  acid  oxidative  decomposition  in  liver.  It  includes

Acetoacetic acid (AcAc) and β- Hydroxybutyric acid (BOH) and acetone. The amount of acetone in ketone body is very small, and it is absorbed immediately. AcAc and BOH is oxidized in extrahepatic tissue through  blood  flow.  The  citric  acid  cycle  provides  more  energy  for  those  tissues,  such  as  bone, myocardium and renal cortex.

At pH 7.0 and 37℃, β- Hydroxybutyrate dehydrogenase (HBDH) catalyzes the dehydrogenation of BOH to produce phthalic acid, and NAD+ is reduced to NADH. At pH 8.8 and 37℃, HBDH reduced AcAc to 3- hydroxybutyrate  or  decarboxylated  to  acetone,  and  NADH  was  oxidized  to  NAD+ .  NADPH  has  a characteristic absorption peak at 340nm. The content of BOH and AcAc can be calculated by detecting the change of absorbance at 340nm. Then the content of ketone body in the sample can be calculated.

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