β-hydroxylic acid (β-HB), in patients with severe acid poisoning, due to increased NADH in the patient in patients, thereby promoting β-hydroxyacetic acid with acetoacetic acidThe ratio is from normal 2: 1 to 16: 1.β-hydroxylic acid is significant in the treatment of diabetic ketoacidosis, and it is also important for the early diagnosis of diabetes.This kit is suitable for serum, plasma, urine, etc. Sbooks.

Under the conditions of pH 8.8 and 37 ° C, β-HB is produced by hydroxyacethase (HBDH) catalyzed by β-hydroxybutyrate, and NAD + is reduced to NadH; in 1-Under the action of MPMS, WST-1 can be reacted with NADH to produce water-soluble formazan, and there is a feature absorption peak at 450 nm.By detecting the wavelength change of 450 nm, the content of β-HB can be calculated.

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