Meaning Significance:

Mitochondrial complex V is also known as F1F0-ATP synthase, widely present in mitochondria in animals, plants, microorganisms, and cultured cells, by F1Composed with F0 two subunits.The enzyme utilizes the proton electrochemical gradient produced by the breathing chain to catalyzes the ATP synthesis, or the process can also be inversely hydrolyzes ATP.In addition, the complex V is also present in the chloroplast, a human bacterium, and a photosynthetic bacterium.The complex V is a key enzyme of mitochondria phosphorylation and chloroplast photosyphosphorylation synthesis ATP. Determination Principle:

Composite V hydrolyzed ATP produces ADP and PI to determine complex V activity by measuring the PI increase rate.

Instruments, consumables: visible spectrophotometer / enzyme, desktop centrifuge, water bath, adjustable pipette, trace quantum-based cattle / 96-well plate,Drug, ice and distilled water.

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