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α-Ketoglutarate HPLC Assay kit
α-Ketoglutarate HPLC Assay kit
α-酮戊二酸含量检测试剂盒 high performance liquid chromatography
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Product Instruction:

α-ketaric acid is one of two types of keto-based derivatives of glutaic acid . Its anion, α-ketoxate is an important bio compound. It is a keto acid product of glutamate deactivated and is an intermediate product of tricarboxylic acid cycles.

α-ketaric acid has an absorption peak at 210 nm, and the content thereof can be determined using HPLC.

 

 

Instruments and reagents required in the test

: High performance liquid chromatograph (Polaris C18-A chromatography Column (4.6 × 250 mm), UV detector (VWD)), desktop centrifuge, adjustable pipette, mortar / homogenizer, EPT (2 mL), needle filter (water system), syringe, pumping Filter, filter (water, organic system), brown into S bottles (50, 1.5 mL), super pure water.

Product content:

 

Extract: liquid 80 ml × 1 bottle, 4 ° C preserved.

Reagent 1: Liquid 2  ML  × 1 bottle, 4 ° C. Reagent 2: Powder × 2 bottles, 4 ° C preserved.

Standard: Powder × 1 bottle, 4 ° C protected from light. 2 ml of distilled water was added to the pre-use to prepare a standard solution of 10 mg / mL alpha-ketoctorate, and stored at 4 ° C to avoid direct sunlight.

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