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Lactic Acid HPLC Assay kit
Lactic Acid HPLC Assay kit
乳酸含量检测试剂盒 high performance liquid chromatography
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Product instruction:

L-lactic acid is based on

corn starch

, which is formed by biological fermentation. An organic acid, a colorless clarified viscous liquid, an aqueous solution acidic reaction . With water, ethanol or diethyl ether can beyond mixed, insoluble in chloroform. Because of its left-handed characteristics, it has good biological hybrid, which can be combined with mammals, can be directly involved in human metabolism, no side effects, are widely used in food, medicine and other fields. L-lactic acid has an absorption peak at 210 nm, which can be determined by HPLC. \u0026 nbsp;

Instruments and reagents needed in the test

:

High performance liquid chromatograph (Polaris C18- A column (4.6 × 250 mm), ultraviolet detector (VWD)), desktop centrifuge, adjustable pipette, mortar / homogenizer, EPT (2 mL), needle filter (water system), syringe , Filter, filter (water, organic system), brown into S bottles (50, 1.5 mL), super pure water. \u0026 nbsp;

Product content:

Extract: liquid 80ml

\u0026 nbsp; × 1 bottle, 4 Save at ° C.

Reagent 1: Liquid 2 \u0026 NBSP; ML \u0026 NBSP; × 1 bottle, 4 ° C.

Reagent 2: Powder × 2 bottles, 4 ° C preserved. Standard: Powder × 1 bottle, 4 ° C protected from light. 2 ml of distilled water was added to prepared

10

Mg / ml L-lactic acid standard solution, and the seal was stored at 4 ° C to avoid direct sunlight.

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