Measurement Significance: xylanase (EC 3.2.1.8) is mainly produced by microorganisms, catalyzes hydrolysis of xylosans, also known as phenylganase or hemicellulase. , Can decompose the cell wall and β-glucan in the brewed or feed industry, reduce the viscosity of materials in the brewing, promote the release of effective substances, and reduce non-starch polysaccharides in the feed, promote the absorption and utilization of nutrients, and thus extensive BAX is generally separated from a microorganism that is generally 9 -11 from the most suitable growth pH.

Determination Principle: Bax Catalyzes xylosan in an alkaline environment into reducing oligosaccharide and monosaccharide, and develops a developing reaction with 3,5-dinitrogen boife under boiling water bath. At 540 nm, there is a feature absorption peak, and the depth of the reaction liquid color is proportional to the amount of reduction glycocharger produced by the enzymatic epoxine, and Bax viability can be calculated by measuring the rate of reactive solution at 540 nm absorbance value.

Required instruments, supplies: balance, low temperature centrifuge, constant temperature water bath, visible spectrophotometer / enzyme symbol, trace quantum-based cuvette / 96-well plate and distilled water.

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