Measurement principle:

GCS catalyzed UDPG and glucose residue generated glycogen and UTP, pyruvinacidase and lactate dehydrogenase further catalyze NADH oxidation to form NAD +,The NADH decline rate was determined at 340 nm, and GCS activity can be reflected.

Instruments, consumables:

Spectrophotometer / enzyme label, desktop centrifuge, adjustable pipette, trace quantine-based cuvette / 96-well plate, mortar, iceAnd distilled water

Means meanive significance:

GCS (EC 2.4.1.11) Catalytic UDPG and glucose residues to produce glycogen and UTP, and extend the sugar chain with alpha-1,4-glycosidial linkage.It is a restriction enzyme of liver and intramuscose, is the main target enzyme of insulin, and has an important role in the regulation of sugar metabolism and the maintenance of blood glucose steady state.

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