Measurement Significance: TH is located on the inner membrane of the mitochondrial, also known as mitochondrial complex six, catalyzes NADH + NADP + and NAD + + NADPH to transform each other, regulate mitochondrial NAD (H) andNADP (H) balance.Reacting the reverse reaction is called TH-2, catalyzed NADPH and NAD + to generate NADP + and NADH.

Measurement principles: NADH and NADPH are characterized by a characteristic absorption at 340 nm, so the catalytic hydrogen reaction cannot cause a change in 340 nm absorbance.Substituted by 3-acetylpyridin adenine dinucleotide (APAD +), the APADH has a characteristic light absorption, and the increase rate of 375 nm light absorption is measured at 375 nm to calculate TH-.2 activity.

Need instruments, consumables: visible spectrophotometer, desktop centrifuge, water bath, adjustable pipette, 1ml glass cuvette, mortar, ice and distilled water.

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