Measurement Significance: S-Ai catalyzes sucrose under pH of 4.5 to 5.0 (acidic) into fructose and glucose, which is one of the key enzymes of soil microbial sucrose metabolism.

As the principle of assay: S-AI catalyzes sucrose degradation to produce reactor sugar, further react with 3,5-dinitrogen-bicylic acid, generate brown red amino compounds, and characterized by 510 nm, in a certain rangeThe 510 nm light absorption increase rate is proportional to the AI activity.

Required instruments, consumables: visible spectrophotometer / enzyme label, desktop centrifuge, water bath, pipette, trace quantum-based cuvette / 96-well plate, mortar, ice and distilled water.

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