Measurement Significance: Soil hydroxylamine reductase can reduce the hydroxylamine formed in the either nitrogen metabolism in the soil as ammonia, and the reducing compound in the soil can be used as a hydrogen supply,Strongly affect the loss of ammissa volatilization during soil nitrogen metabolism, indirectly affect the efficiency of nitrogen fertilizer.

Determination principle: FE3 + in ammodium sulfate can oxidize hydroxylamine into nitrogen, and it is reduced to Fe2 +, Fe2 + to form an orange-red complex with the adjacent Philoline under weak acid conditions, and absorbing peak at 510 nm.Hydroxylamine reductase acts on hydroxylamine, reduces the amount of complex formation, and reduces the absorber reductase activity in 510 nm.

Instruments, consumables: balance, normal temperature centrifuge, visible spectrophotometer / enzyme symbol, trace quantity-free cuvette / 96-well plate, oscillator, nitrogen blower.

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