Measuring significance: sorbitol is widely present in animals, plants, microorganisms, and cultured cells, not only one of the form of sugar transport, but also closely related to biological stress and food flavor.Therefore, sorbitol content changes in sugar metabolism, anti-counterpart and food research.

Measurement principle: sorbitol forms a blue complex in an alkaline solution with copper ions, and has a characteristic absorption peak at 655 nm wavelength.

Need instruments, consumables: visible spectrophotometer / enzyme label, water bath, pipette, trace quantine-based cuvette / 96-well plate, mortar, ice and distilled water.

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