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Measurement Significance: Hepaticase is a fat alleled enzyme, synthesized in liver substantive cells, is present in the surface of the liver sinus gap endothelial cell surface and the sinus gap chamberThe surface can promote the transformation of the cholesterol in the lipoprotein to the steroid hormone, and the plasma in the plasma can cause the plasma to increase the LDL level, accelerate the occurrence of arterial porridge S.
Determination principle: Hepatic esterase hydrolyzed alpha-acetate produces alpha-naphthol, can form a purple red azo compound with solid blue B salt, and there is a characteristic absorption peak at 595 nm, and the color is deep in a certain range.Positively correlated with hepaticase activity.
Need instruments, consumables: balance, frozen centrifuge, mortar, water bath, visible spectrophotometer / enzyme symbol, trace quantum-based cuvette / 96-well plate.
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