Meaning Significance: GCDH (EC 1.1.1.47) catalyzes D-glucose and NAD (P) to form D-glucoic acid and NAD (P) h, and there are a large number of liver present in higher animals.And weak oxidation of vinegar.The GCDH is used in oliguous fructose production not only removes glucose in low polyggate, not only the content of oligo fruit sugar, but also the resulting gluconic acid with calcium ions, calcium calcium

assay: GCDH catalysisD-glucose and NAD generate D-gluconic acid and NADH, and the NADH rise rate can be reflected in 340 nm, which can reflect the GCDH activity.

Need instruments, supplies: UV spectrometer / enzyme label, desktop centrifuge, adjustable pipette, trace quantine-based cuvette / 96-well plate, mortar, ice and distilled water.

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