Determination of significance: glucoamylase, i.e., glucoamylase (EC 3.2.1.3), also known as γ- amylases, is an exo-glycosidases, which from the starch hydrolysis of non-reducing terminal α-1,4 glucosidic bonds and α-1,6 glycosidic bonds, completely hydrolyzed starch to glucose, and therefore widely used in alcohol, liquor, antibiotics, amino acids, organic acids, glycerol, starch, sugar industry in, it is one of my country's important industrial enzymes.

Measurementprinciple:glucoamylasehydrolysis of solublestarchto glucose,and thered-browncompound of3,5-dinitrosalicylic acidgeneration,lightabsorptionmaximumat 540nm,the reactionsolutioncolor shadeswithin a predetermined rangeandproportional to the amountof glucose,the measurementmay becalculatedglucoamylasevitality.

needs, consumables:balance,low temperaturecentrifuge,mortar,Vis/microplate reader,microquartzcuvette/96,constant temperature water bath.

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