Measurement:

Multi-galacturoxidase (EC3.2.1.15) is a cell wall binding protein that can catalytic pectin molecules α-(1, 4) - Cracking of polyallacturonic acid, participate in pectin degradation, result in solving the cell wall structure, resulting in fruit softening, ripening, leaf and flowers, pathogen defense, cell stretch development and wooding relatedIt has a high study value in the field of plant disease resistance and food storage.\u0026 nbsp; Principles:
Multi-galacturonic acid solution acid generate galacturonic acid, has a reducing aldehyde group, reacting with DNS reagent to form red-brown substance, characterized at 540 nmThe peak of the absorption peak was determined at 540 nm, and the variation of the absorbance value at 540 nm can be calculated to be polylactonicuronicase activity.\u0026 nbsp;
Self-helicable experimental supplies and instruments: balance, low temperature centrifuge, visible spectrophotometer, 1 ml glass cuvette, constant temperature water bath.\u0026 nbsp;

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