Measurement Significance: xylanase (EC 3.2.1.8) is mainly produced by microorganisms, catalyzes hydrolysis of xylosans, also known as phenylganase or hemicellulase. , Can decompose the cell wall and β-glucan in the brewed or feed industry, reduce the viscosity of materials in the brewing, promote the release of effective substances, and reduce non-starch polysaccharides in the feed, promote the absorption and utilization of nutrients, and thus extensive Based on brewing and feed industries, ACX generally separates acid fungi, bacteria, and partial mold.

Determination principle: CX can degrade xylosaccharide into a reducing oligosaccharide and monosaccharide in an acidic environment, and develop color reacts with 3,5-dinitrogen-bicylic acid under boiling bath At 540 nm, there is a feature absorption peak, and the depth of the reaction liquid color is proportional to the reduction glycoconity generated by the enzymatic hydrolysis, and the ACX viability can be calculated by measuring the rate of reaction of the reaction liquid at 540 nm absorbance value.

Required instruments, supplies: balance, low temperature centrifuge, constant temperature water bath, visible spectrophotometer / enzyme symbol, trace quantum-based cuvette / 96-well plate and distilled water.

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