Measurement Significance: α-Gal (EC 3.2.1.22) is widely used in animals, plants, microorganisms, and cultured cells, which can catalyze hydrolysis of alpha galactoside linkage.It is mainly involved in the degradation of galactoside such as cotton candy, hydraulose sugar, honey diose and galactomannan.α-gal is essential for the germination of plant seeds. The initial seed germination, its catalytic D-galactose quickly converted and consumed through the glycolysis pathway, providing the initial source of energy for seed germination, and later participated in cell wall storagePolysaccharide hydrolysis.

Measurement principle: α-Gal decomposition pair-nitrobenzene-α-D-pyranium galactoside to-nitrophenol, the latter has the maximum absorption peak at 400 nm, and elevated by measuring the absorption valueRate to calculate α-GAL activity.

Need instruments, consumables: visible spectrophotometer / enzyme label, desktop centrifuge, water bath, adjustable pipette, trace quantum-based cuvette / 96-well plate, mortar, ice and distilled water.

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