Measurement:

GAPDH catalyzed 3-phosphoride oxidation into 1,3-dithiosphate, is a key enzyme of glycolysis pathway, with sugarDisease pathways, maintenance of blood glucose concentration in the body is closely related to diabetes, and play an important role in body sugar, lipid, and protein metabolism. As determined:

3-Phosphate kinase catalytic triphosphate acid and ATP formation of 1,3 diphosphate.GAPDH reverse catalytic 1,3 dikhaloic acid and NADH to form 3 phosphorched aldehyde, inorganic phosphorus and NAD, and the reduction in NADH can reflect the high and low of GADPH activity.

Instruments, consumables: Spectrophotometer / enzyme label, constant temperature water bath, desktop centrifuge, adjustable pipette, trace quantum-based cuvette / 96-well plate,Drug, ice and distilled water.

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