Phosphoenolpyruvate carboxylase (PEPC) Activity Assay Kit 
Note: It is necessary to predict 2-3 large difference samples before the formal determination.        
Operation Equipment: Spectrophotometer/microplate reader

Cat No: AK0242

Product Description:

Phosphoenolpyruvate carboxylase (EC 4.1.1.31) is widely found in plants and microorganisms. It catalyzes the irreversible reaction of phosphoenolpyruvate and carbon dioxide to oxaloacetic acid. It is also a key enzyme for C4 plants and cam plants to fix CO2 . It plays an important role in regulating the operation of tricarboxylic acid cycle.

PEPC can catalyze phosphoenolpyruvate and carbon dioxide to form oxaloacetate and HPO42- . Malate dehydrogenase can catalyze oxaloacetate and NADH to produce malate and NAD+ . The rate of NADH reduction was measured at 340 nm and PEPC activity was calculated.

 

 

 

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