Measurement Significance: ICl (EC4.1.3.1) mainly exists in plant and microorganisms, oil crop seeds in the germination process, transforming fat through the acethemical cycle and other processesCurable carbohydrate.ICL is one of the key enzymes of acetalic acid cycle.

Determination principle: ICl catalyzed is degraded into acetalic acid and succinic acid, glyoxylic acid, and NADH to form ethanol and NAD, NadH in 340 nm, and Monitor 340 nm absorbance.Reduce the rate of ICL indirect reactions.

Need instruments, supplies: UV spectrophotometer / enzyme label, desktop centrifuge, water bath, pipette, trace quantum-based cuvette / 96-well plate, mortar, ice and distilled water

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