Measurement Significance:

AKP refers to an enzyme that catalyzes a protein peptide hydrolysis under alkaline conditions, belonging to a serine protease.In addition, the enzyme is also capable of hydrolyzing ester bonds, amide bonds, function of transfection and peptide.This enzyme is one of the main industrial enzymes, widely used in pharmaceutical, silk, food, tanning and other industries. Determination Principle: Under alkaline conditions, AKP aqueous precipitin produces tyrosine; under alkaline conditions, tyrosine reductance phosphomolybdates are used for tungsten blue; tungsten blue is characterized by 680 nmAKP activity is calculated to calculate the AKP activity of the absorption peak.
Required instruments and supplies: Visit spectrophotometer, water bath, magnetic stirrer, adjustable pipette, 1.5 mL EP T, 1 mL glass cuvette and distilled water.

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