Measurement Significance: ADH is a key enzyme of short-chain alcohol metabolism in the biological body, catalytic ethanol and acetaldehyde reversal, play an important role in many physiological processes.Mammalian ADH is mainly in the liver, and liver damage causes ADH release to serum.Serum ADH activity high reflects whether hepatic function is abnormal.

Determination principle: ADH catalytic NADH reducing acetaldehyde to generate ethanol and NAD +, NADH has absorption peak at 340 nm, and NAD + is not; determined 340 nm absorbance drop rate to calculate the ADH activity.

Required instruments, consumables: mortar, ice, low temperature centrifuge, ultraviolet spectrophotometer / enzyme label, trace quantity-free cuvette / 96-well plate, adjustable pipette and distilled water.

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