Note: Select 2-3 Expected Differences of S this predicted before formally measured. \u0026 Nbsp;

Product Instruction: adenosine monophosphate (AMP) exists widely in animals, plants, microorganisms and cultured cells are in vivo by the ATP and It formed after release energy ADP. Phosphate-binding group can continue to form adenosine diphosphate (ADP) and adenosine triphosphate (ATP). ATP is not fully hydrolyzed product. The AMP absorption peak at 254 nm, the content can be measured using HPLC. \u0026 Nbsp;

equipment and reagents required for the test: HPLC (C18 column (4.6 × 250 mm), UV detector (VWD) ), desktop centrifuge, adjustable pipette gun, a mortar / homogenizer, brown EP T, syringe filters (50, aqueous, 0.45 μm), a syringe, a suction filter, the filter membrane (organic, water) , S into brown bottles (50, 2 mL), acetonitrile (HPLC grade, 500 mL), ultrapure water. \u0026 nbsp; Product content: \u0026 nbsp;

extract 1: liquid 80 mL × 1 bottle, 4 ° C save. \u0026 Nbsp;

Extract II: Liquid 40 mL × 1 bottle, stored 4 ℃.

\u0026 nbsp; Reagent: 15 mL × 1 bottle of liquid, stored 4 ℃. Before use to take a 3.5 mL reagent was added to 1000 mL of ultrapure water, adjusted for two reagent pH \u003d 6.15, a flow phase B, a sealing. \u0026 Nbsp;

Reagent II: liquid 5 mL × 1 bottle, stored 4 ℃. \u0026 Nbsp;

Standard: powder branched × 1, -20 ℃ stored. Was added 2.0 mL distilled water to 1 μmol / mL AMP standard solution before use, frozen -20 ℃. In order to ensure the integrity of AMP, avoid repeated freezing and thawing.

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