Measurement Significance: NR is widely existing in plants, is a key enzyme that transforms plant nitrate nitrogen into ammonia nitrogen is also induced by induce enzymes.And quality has an impact. As determined:
NR catalyzed nitrate was reduced to nitrite, NO3 ˉ + NADH + H + → NO2 ˉ + NAD + + H2O; the resulting nitrite can be under acidic conditions, to aminobenzenesulfurAcid and α-naphthyl amines were quantified to form a red azo compound; the resulting red azo compound had maximum absorption peaks at 340 nm, which can be determined by spectrophotometry. Instruments and reagents required:
Visible spectrophotometer, water bath, desktop centrifuge, adjustable pipette, 1 ml glass cuvette, mortar, ice and distilled water.

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