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Measurement Significance: Aldehyde dehydrogenase (EC 1.2.1.10) is one of aldehyde dehydrogenase, widely present in various animals, plants and microorganisms.The main role is to oxidize the Aldehyde into acetic acid, which plays a major role in alcohol metabolism.In humans and many other animals, mitochondrial Aldehyde dehydrogenase transforms alcohol-like alcohols detrimental to organisms, so the Aldehyde dehydrogenase is highly concerned in cell detoxification research; at the same time, Aldehyde dehydrogenase is in molecular biology.And there is a wide range of research applications in terms of testing.
As determined: Under the conditions of coenzyme I present, Aldehyde dehydrogenase catalyzes acetaldehyde and NAD + to acetic acid and NADH, and the absorbance value at 340 nm increases, and the light absorbance value at 340 nm is determined.The activity of the Aldehyde dehydrogenase can be calculated.
Instruments, consumables: balance, centrifuge, UV spectrometer / enzyme symbol, trace quantity, distilled water, distilled water.
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