Measurement: Blood phosphorus mainly refers to inorganic phosphorus salt in the form of inorganic phosphorus salt. The relationship between calcium and phosphorus concentration in plasma. When the Mg / DL is indicated, the product of the two is 30 to 40. When ([Ca] × [P]) \u0026 gt; 40, calcium and phosphorus were deposited in bone tissue in the form of bone salts. If ([CA] × [P]) \u0026 lt; 35 hinders the calcification of the bone, even the bone salt can be dissolved, affecting the bone effect. The relative stability of blood calcium and phosphorus content is dependent on calcium, phosphorus absorption and excretion and calcification and two metabolism of decimination. The above equilibrium is adjusted by hormone such as vitamin D3, parathyroid and calcitonin. Determination principle:

After removing organic phosphorus in serum, the inorganic phosphorus salt produces phosphomolybdic acid with ammonium molybdate, which is blue by ferrous sulfate. Light absorption at 660 nm; the phosphorus content in the blood is calculated by measuring 660 NM absorbance.

Sheets :

:

: : Variable spectrophotometer, centrifuge, adjustable pipette, 1 ml glass cuvette and distilled water. Hemophosphorus concentration measurement operation:

1. The spectrophotometer is preheated by 30 min or more, and the wavelength is adjusted to 660 nm, and the distillation water is adjusted. 2. Take out the standard liquid and thaw room temperature. 3. Serum pretreatment: 50 μL of serum, add 950 μl of reagent, mix resiliency 8000 rpm, centrifuge for 10 min, take the supernatant, to be tested.

4. Blank T: Take 1 ml of glass cuvette, 250 μl of distilled water, 250 μL of the reagent 1, 500 μL of reagent three, mixed after 10 min, and measured the absorbance in 660 nm to be described as a blank T.

5. Standard T: Take 1 ml of glass cuvette, sequentially add 250 μl of standard liquid, 250 μL of reagent 1, 500 μL of reagent three, mixed back to 10 min, and measure the absorbance in 660 nm as a standard T.

6. Determination T: Take 1 ml of glass cuvette, sequentially add 250 μl of supernatant, 250 μL of reagent 1, 500 μL of the reagent three, retrapate after mixing 10 min, at 660 nm measurement absorbance is measured as a Determination T .

Calculation of phosphorus:

Blood phosphorus content (m mol / dl) \u003d [C standard liquid flat × (A determination TA blank T) ÷ (A standard TA blank T)] × S product dilution multiples × vs total \u003d 0.04 × (a measure TA blank T) ÷ (a standard TA blank T) C standard liquid: 0.02 mmol / L; S product dilution multiple: (50 μL serum + 950 μL reagent 1) ÷ 50 μL serum \u003d 20: VS Total:100 ml \u003d 0.1 L.

1, the reagent three needs to be prepared before it, if not used, 4 ° C save, up to 3 days.

2, during the measurement process, hemolysis should be avoided, because the organic phosphorine can be enzymated by the enzymatic hydrocephalus after entering the serum, which increases the content of serum inorganic phosphorus.

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