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Meaning significance: mitochondrial complex IV is also known as cytochrome C oxidase, is also a common component of the main path and branch of mitochondrial breathing electron transfer chain, responsible for catalysisThe oxidation of the reduced cytochrome C and finally transmits electrons to oxygen and produces water.
Determination principle: Reducing type cytochrome C is characterized by characterized light absorption in 550 nm, mitochondrial complex IV catalytic reduction type cytoside C to form oxidation cytosol C, so 550 nm light absorption fell rate can reflect mitochondrialComplex IV activity. Required instruments and supplies:
Visit spectrophotometer, desktop centrifuge, water bath, adjustable pipette, 1ml glass cuvette, mortar, ice and distilled water.
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