Measurement Significance: FDA hydrolysis reaction can be well reactive soil change in soil changes and conversion of organic matter in ecosystems, is soil quality researchOne of the important biological indicators.

As the principle:

FDA is a colorless compound that can be hydrolyzed by many soil enzymes in the medium, and is dehydrated, and the fluorescein of the enzymolysis is produced, and it is difficult to stabilize.Decomposed and has a strong absorption peak at 490 nm, and the FDA hydrolyzed enzyme activity can be calculated by detecting a change in the absorbance value at 490 nm. Instruments, consumables:

balance, low temperature centrifuge, visible spectrophotometer / enzyme label, trace quantum-based cuvette / 96-well plate, constant temperature water bath.

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