Measuring significance:

Reducing sugar is widely present in animals, plants, microorganisms and cultured cells.Reducing sugar in the plant mainly includes glucose, fructose and maltose, etc., is the most common monosaccharide and double sugar, where glucose and fructose are not only the main substrate of breathing, but also the substrate of sucrose, starch and cellulose. Measurement principle:

Heating to promote the 3,5-dinitrogenated salicylic acid solution in the alkaline solution and the reducing sugar to form brown red amino compounds, and there is a characteristic absorption peak at 540 nm;Within the concentration range, the reductant sugar content is linear with 540 nm absorbance, and the amount of reducing sugar in S is obtained according to standard curves.

Instruments, consumables: visible spectrophotometer / enzyme label, water bath, adjustable pipette, trace quantine-based cuvette / 96-well plate, mortar, distilled water.

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