Measurement Significance: AGP (EC 2.7.7.21) mainly exists in plants, catalyzed a direct precursor ADPG of glucose-1-phosphate and ATP to form starch synthesis, is a plant starch organismSynthetic main speed step

Measurement principle: AGP catalyzed reverse reaction to form G1P, a phosphate denominase and 6-phosphate dehydrogenase added in the reaction system to form 6-phosphate glucoseAcid and NADPH, the NADPH increase rate was determined at 340 nm to calculate the AGP activity.

Need instruments, supplies: UV spectrophotometric / enzyme label, water bath, desktop centrifuge, adjustable pipette, trace quantine-based cuvette / 96-well plate, mortar, ice and distilled water

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