Total cholesterol (TC) Content Assay Kit

Note: Take two or three different samples for prediction before test.

Operation Equipment: Spectrophotometer/microplate reader

Catalog Number: AK0326

Product Description

Total cholesterol (TC) refers to the total cholesterol of all lipoproteins. It includes free cholesterol and cholesterol esters.

Esterase can catalyze the hydrolysis of cholesterol ester to produce free cholesterol (FC) and free fatty acid (FFA), thus transforming cholesterol ester into FC; Furthermore, cholesterol oxidase can catalyze FC to form Δ4-cholesterone and H2O2 ; Finally, peroxidase can catalyze the oxidation of 4-aminoantipyrine and phenol by H2O2 to form red quinones. It has a characteristic absorption peak at 500 nm, and its color depth is directly proportional to TC content.

 

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