Product Instruction  

Detection Method	HPLC
Specification	50T/48S

Product Instruction：
β-胡萝卜素是类胡萝卜素之一，是一种橘黄色脂溶性化合物，普遍存在于自然界中，也是最稳定的天然色素。许多天然食物中例如：绿色蔬菜、甘薯、胡萝卜、菠菜、木瓜、芒果等，皆存有丰富的β-胡萝卜素。
β-胡萝卜素在450 nm下有吸收峰，可以利用HPLC测定其含量。

试验中所需的仪器和试剂：
高效液相色谱仪（C18柱（4.6×250 mm），紫外检测器（VWD））、台式离心机、可调式移液枪、研钵/匀浆器、EPT、针头式过滤器（50个，有机系，0.45 µm），注射器，抽滤器，滤膜（有机系），棕色进S瓶（50个，1.5 mL）、甲醇（色谱纯）、超纯水、二氯甲烷（色谱纯）。

产品内容：
提取液：液体60 mL ×1瓶，4℃保存。
标准品：粉剂×1支，-20℃避光保存。临用前加入1 mL二氯甲烷配制成2 mg/mL β-胡萝卜素标准溶液，-20℃密封保存，避免阳光直射。

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