Significance: SSS (EC 2.4.1.21) usually exists in plastid matrix as a free state, catalyzes starch chain elongation, and is mainly responsible for amylopectin synthesis.

Determination principle: SSS catalyzes the reaction of ADPG with starch primer (glucan), transfers glucose molecules to starch primer, and generates ADP at the same time. Pyruvate kinase, hexokinase and 6-phosphoglucose dehydrogenase added to the reaction system catalyze the reduction of NADP+ to NADPH in turn, and the amount of NADPH production is proportional to the amount of ADP production in the previous step. SSS activity can be calculated by measuring the increase of NADPH at 340nm.

Equipment needed, supplies: UV spectrophotometer/microplate reader, water bath, table centrifuge, adjustable pipette, microquartz cuvette / 96-well plate, mortar, ice and distilled water.

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